| 產品編號 | bsm-61678R |
| 英文名稱 | CD166 Recombinant Rabbit mAb |
| 中文名稱 | 活化白細胞粘附分子重組兔單抗 |
| 別 名 | ALCAM; CD166; MEMD. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 7E3 |
| 交叉反應 | Human,Mouse,Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1:20-50,IP=1:20-50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 65 kDa |
| 檢測分子量 | 100 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human ALCAM: 540-583/583 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. SWISS: Q13740 Gene ID: 214 ALCAM/CD166是免疫球蛋白超家族的成員,對多種腫瘤生長和轉移相關的腫瘤細胞特性具有調節(jié)作用,ALCAM/CD166在乳腺癌、前列腺癌、食道癌、結腸癌及惡性黑色素瘤等腫瘤都有一定的表達，尤以結腸、直腸癌表達顯著。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD166 monoclonal antibody, unconjugated (bsm-61678R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
