| 產品編號 | bs-0553R |
| 英文名稱 | Collagen VI alpha 1 Rabbit pAb |
| 中文名稱 | 抗Ⅵ型膠原抗體 |
| 別 名 | CO6A1_HUMAN; COL6A1; COL6A2; COL6A3; Collagen alpha 1(VI) chain; Collagen alpha 1(VI) chain precursor; Collagen alpha-1(VI) chain; Collagen type VI alpha 1; Collagen type VI alpha 2; Collagen type VI alpha 3; Collagen VI alpha 1 polypeptide; Collagen VI alpha 2 polypeptide; Collagen VI alpha 3 polypeptide; CollagenVI; Human mRNA for collagen VI alpha 1 C terminal globular domain; OPLL; PP3610. |
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Specific References (3) | bs-0553R has been referenced in 3 publications.
[IF=1.837] Yang Zhang. et al. Plumbagin Inhibits Proliferation, Migration, and Invasion of Retinal Pigment Epithelial Cells Induced by FGF-2. Tissue Cell. 2021 Oct;72:101547 WB ; Human.
[IF=1.794] Gao X et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020 Mar;216(3):152831. IHC-P ; Human.
[IF=1.785] Xiaoyan Yu. et al. Ginkgo?biloba leaf extract prevents diabetic nephropathy through the suppression of tissue transglutaminase. Exp Ther Med. 2021 Apr;21(4):1-1 ELISA ; Rat.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 106 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Collagen VI: 951-1028/1028 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq, Jul 2008] Function: Collagen VI acts as a cell-binding protein. Subunit: Trimers composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). Subcellular Location: Secreted, extracellular space, extracellular matrix (By similarity). Tissue Specificity: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. DISEASE: Bethlem myopathy (BM) [MIM:158810]: A benign autosomal dominant proximal myopathy characterized by early childhood onset and joint contractures most frequently affecting the elbows and ankles. Note=The disease is caused by mutations affecting the gene represented in this entry. Ullrich congenital muscular dystrophy (UCMD) [MIM:254090]: UCMD is a congenital myopathy characterized by muscle weakness and multiple joint contractures, generally noted at birth or early infancy. The clinical course is more severe than in Bethlem myopathy. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the type VI collagen family. Contains 3 VWFA domains. SWISS: P12111 Gene ID: 1293 Database links: Entrez Gene: 1291 Human Entrez Gene: 1292 Human Entrez Gene: 1293 Human Entrez Gene: 12833 Mouse Entrez Gene: 12834 Mouse Entrez Gene: 12835 Mouse Omim: 120220 Human Omim: 120240 Human Omim: 120250 Human SwissProt: P12109 Human SwissProt: P12110 Human SwissProt: P12111 Human SwissProt: Q02788 Mouse SwissProt: Q04857 Mouse Unigene: 474053 Human Unigene: 2509 Mouse Unigene: 232118 Rat |
| 產品圖片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Collagen VI alpha 1 (bs-0553R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:106 kD
Observed band size: 131 kD
Sample:
Lane 1: Ovary (Mouse) Lysate at 40 ug
Lane 2: Placenta (Mouse) Lysate at 40 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: Ovary (Rat) Lysate at 40 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Lane 6: U2os (Human) Cell Lysate at 30 ug
Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-Collagen VI alpha 1 (bs-0553R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 109 kD
Observed band size: 125 kD
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Collagen VI alpha 1) Polyclonal Antibody, Unconjugated (bs-0553R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: The skin of the infant; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen-VI-alpha1 Polyclonal Antibody, Unconjugated(bs-0553R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
