| 產(chǎn)品編號 | bs-0428R |
| 英文名稱 | Rabbit Anti-DISC1 (NT) antibody |
| 中文名稱 | DISC1 N端抗體 |
| 別 名 | disrupted in schizophrenia1; C1orf136; KIAA0457; RP4-730B13.1; SCZD9; DISC1; DISC1_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 92kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DISC1: 101-200/854 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
DISC1 (disrupted in schizophrenia 1) is a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. DISC1 is a multifunctional protein that is involved in neurite outgrowth and cortical development, through its interaction with proteins of the centrosome and cytoskeletal system, and is implicated in schizophrenia. Regions of the primate brain which express DISC1, including the hippocampus, lateral septum, amygdala, cerebral cortex, cerebellum and paraventricular hypothalamus, are reported to be involved in schizophrenia in humans.
Function: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation. Subcellular Location: Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Colocalizes with NDEL1 in the perinuclear region and the centrosome (By similarity). Localizes to punctate cytoplasmic foci which overlap in part with mitochondria. Colocalizes with PCNT at the centrosome. Tissue Specificity: Ubiquitous. Highly expressed in the dentate gyrus of the hippocampus. Also expressed in the temporal and parahippocampal cortices and cells of the white matter. DISEASE: Note=A chromosomal aberration involving DISC1 segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Translocation t(1;11)(q42.1;q14.3). The truncated DISC1 protein produced by this translocation is unable to interact with ATF4, ATF5 and NDEL1. Genetic variation in DISC1 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. SWISS: Q9NRI5 Gene ID: 27185 Database links: Entrez Gene: 27185 Human Omim: 605210 Human SwissProt: Q9NRI5 Human Unigene: 13318 Human DISC1基因是蛋白質(zhì)復(fù)合體中的一部分,它對大腦皮層發(fā)育過程中細(xì)胞的正常運動很重要。 DISC-1基因的變異增加了患精神分裂癥的風(fēng)險。研究人員發(fā)現(xiàn)，抑制老鼠的DISC1活性則改變了動物大腦的發(fā)育，引起了大腦皮層輕微的變異，而在對精神分裂癥病人進行尸檢時在其大腦中見到了此變化。研究人員還發(fā)現(xiàn)將DISC1與對大腦發(fā)育和調(diào)節(jié)神經(jīng)傳遞素水平很重要的分子信號路徑聯(lián)系起來，精神病患者的神經(jīng)傳遞素水平往往是不正常的。 新研究顯示DISC1蛋白質(zhì)與一個叫PDE4B的酶相互作用，而影響信號傳遞分子cAMP的活性。這個信號傳遞系統(tǒng)過去在其它試驗系統(tǒng)中被發(fā)現(xiàn)與學(xué)習(xí)、記憶、以及情緒有關(guān)，這與該系統(tǒng)中的變化可能促成精神分裂癥是一致的。 |
| 產(chǎn)品圖片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-DISC1 (NT) (bs-0428R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 92 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DISC1 (NT)) Polyclonal Antibody, Unconjugated (bs-0428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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