| 產品編號 | bs-0378R |
| 英文名稱 | Runx3 Rabbit pAb |
| 中文名稱 | Runx3抗體 |
| 別 名 | RUNX3_HUMAN; Runt-related transcription factor 3; AML2; CBFA3; PEBP2A3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3(CBF-alpha-3); Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit(PEA2-alpha C; PEBP2-alpha C); SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; |
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Specific References (2) | bs-0378R has been referenced in 2 publications.
[IF=3.48] He, Jian?\Feng, et al. "xpression of RUNX3 in salivary adenoid cystic carcinoma: Implications for tumor progression and prognosis." Cancer science 99.7 (2008): 1334-1340.. IHC-P ; Human.
[IF=2.35] Liu, Yanhua, et al. "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." International Journal of Molecular Medicine (2017). WB ; Human.
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| 研究領域 | 腫瘤 細胞生物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse (predicted: Rat,Cow,Chicken,Dog) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Runx3: 151-250/415 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Subunit: Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. Subcellular Location: Nucleus. Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Post-translational modifications: Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN. Similarity: Contains 1 Runt domain. SWISS: Q13761 Gene ID: 864 Database links: Entrez Gene: 864 Human Omim: 600210 Human SwissProt: Q13761 Human Unigene: 170019 Human 新型抑癌基因/一種新發(fā)現(xiàn)的抑癌基因。經研究發(fā)現(xiàn),在人類胃癌細胞系和胃癌組織中普遍存在Runx3基因表達的缺失或下調,該蛋白與胃癌的發(fā)生、發(fā)展密切相關。 RUNX3基因被認為是一種新發(fā)現(xiàn)的抑癌基因,對胃癌細胞的生長有明顯的抑制作用在胃黏膜上皮細胞調控、對脊神經節(jié)的神經發(fā)育和T細胞分化中都發(fā)揮重要作用。 隨著研究的不斷深入，Runx3蛋白有望成為胃癌診斷的一個新型生物學標志物和基因治療靶點。 |
| 產品圖片 |
Sample: A549 Cell Lysate at 30 ug
Primary: Anti- Runx3 (bs-0378R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Sample: Lymph node (Mouse) Lysate at 30 ug
Primary: Anti- Runx3 (bs-0378R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 44 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Runx3 Polyclonal Antibody, Unconjugated(bs-0378R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
