| 產(chǎn)品編號 | bs-0259R |
| 英文名稱 | heavy chain cardiac Myosin Rabbit pAb |
| 中文名稱 | 心肌肌球蛋白重鏈抗體 |
| 別 名 | MYH6+MYH7; MYH6/MYH7; alpha MHC; ASD3; CMD1S; CMH1; MGC138376; MGC138378; MPD1; MYH 6; MYH 7; MYH6; MYH7; MYHC A; MYHC; MYHC B; MyHC-alpha; MyHC-beta; MYHCA; MYHCB; Myosin heavy chain cardiac muscle alpha isoform; Myosin heavy chain cardiac muscle beta isoform; Myosin heavy polypeptide 7 cardiac muscle beta; MYH6_HUMAN; MYH7_HUMAN; heavy chain cardiac Myosin. |
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Specific References (6) | bs-0259R has been referenced in 6 publications.
[IF=6.656] Xiaoyu Tian. et al. Xinbao Pill attenuated chronic heart failure by suppressing the ubiquitination of β-Adrenergic Receptors. PHYTOMEDICINE. 2023 Apr;:154830
[IF=5.595] Du J, Zhang P et al. MicroRNA-351-5p mediates skeletal myogenesis by directly targeting lactamase-β and is regulated by lnc-mg.(2018) FASEB J. Sep 14 ICC ; Mouse.
[IF=4.49] Gan M. et al. miR-222 is involved in the regulation of genistein on skeletal muscle fiber type. The Journal of Nutritional Biochemistry(2019). ICF ; Mouse.
[IF=3.905] Zhi Hu. et al. MUSTN1 is an indispensable factor in the proliferation, differentiation and apoptosis of skeletal muscle satellite cells in chicken. Exp Cell Res. 2021 Oct;407:112833 WB ; chicken.
[IF=3.585] Lv N et al. Perfluorooctanoic acid-induced toxicities in chicken embryo primary cardiomyocytes: Roles of PPAR alpha and Wnt5a/Frizzled2. Toxicol Appl Pharmacol. 2019 Aug 21;381:114716. ICC ; chicken embryo.
[IF=2.8] Li, Hao, et al. "Perivascular adipose tissue-derived leptin promotes vascular smooth muscle cell phenotypic switching via p38 mitogen-activated protein kinase in metabolic syndrome rats." Experimental Biology and Medicine (2014): 1535370214527903. WB ; Rat.
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| 研究領(lǐng)域 | 心血管 免疫學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 213 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from the middle of human MyHC-beta: 1101-1200/1938 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Mar 2010]. Function: Muscle contraction. Subunit: Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Subcellular Location: Cytoplasm, myofibril. Note=Thick filaments of the myofibrils. DISEASE: Atrial septal defect 3 (ASD3) [MIM:614089]: A congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Note=The disease is caused by mutations affecting the gene represented in this entry. Cardiomyopathy, familial hypertrophic 14 (CMH14) [MIM:613251]: A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Note=The disease is caused by mutations affecting the gene represented in this entry. Cardiomyopathy, dilated 1EE (CMD1EE) [MIM:613252]: A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Note=The disease is caused by mutations affecting the gene represented in this entry. Sick sinus syndrome 3 (SSS3) [MIM:614090]: The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry (PubMed:21378987). The lifetime risk of being diagnosed with sick sinus syndrome is higher for carriers of variant p.Arg721Trp than for non-carriers (PubMed:21378987). Similarity: Contains 1 IQ domain. Contains 1 myosin head-like domain. SWISS: P12883 Gene ID: 4625 Database links: Entrez Gene: 4624 Human Entrez Gene: 4625 Human Entrez Gene: 140781 Mouse Entrez Gene: 17888 Mouse Omim: 160710 Human SwissProt: P12883 Human SwissProt: P13533 Human SwissProt: Q02566 Mouse SwissProt: Q91Z83 Mouse Unigene: 929 Human Unigene: 319176 Mouse Unigene: 127778 Rat 心肌肌球蛋白(cardiac myosin，CM)亦稱肌凝蛋白，它是粗肌絲的主要成分，也是心肌細(xì)胞存在最多的結(jié)構(gòu)蛋白。CM由一條重鏈(MHC)和兩條輕鏈(MLC)兩種亞單位組成，輕鏈分為輕鏈Ⅰ(MLC-Ⅰ)和輕鏈Ⅱ(MLC-Ⅱ)。CM在功能上與骨骼肌相似，但在氨基酸組織及結(jié)構(gòu)上有差別。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-SM-MHC Polyclonal Antibody, Unconjugated(bs-0259R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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