| 產(chǎn)品編號 | bs-9705R |
| 英文名稱 | ITM2A Rabbit pAb |
| 中文名稱 | 完整膜蛋白E25A抗體 |
| 別 名 | BRICD2A; BRICHOS domain containing 2A; E25 protein; E25A; Integral membrane protein 2A; ITM2A; ITM2A_HUMAN; Protein E25. |
| 研究領(lǐng)域 | 細(xì)胞生物 神經(jīng)生物學(xué) 干細(xì)胞 細(xì)胞周期蛋白 細(xì)胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Pig) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 30 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ITM2A: 21-120/263 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The type II integral membrane (ITM2) protein family consists of three members ITM2A (also designated E25), ITM2B and ITM2C. ITM2A expression is high in osteogenic and lymphoid tissues, while both ITM2B and ITM2C are expressed in brain. Mutations in the ITM2B gene can lead to familial British dementia (fbd), and autosomal dominant disease characterized by progressive dementia, spasticity, and cerebellar ataxia, or familial Danish dementia (fdd), an autosomal dominant disorder characterized by cataracts, deafness, progressive ataxia, and dementia. The ITM2A 263-amino acid protein contains an N-terminal cytosolic domain, an uncleaved signal anchor sequence, and a tyrosine-rich C-terminal domain. Human ITM2A shares 91% homology with mouse ITM2A. Subcellular Location: Membrane; Single-pass type II membraneprotein (Potential). Similarity: Belongs to the ITM2 family. Contains 1 BRICHOS domain. SWISS: O43736 Gene ID: 9452 Database links: Entrez Gene: 9452 Human Omim: 300222 Human SwissProt: O43736 Human Unigene: 17109 Human Unigene: 694944 Human |
| 產(chǎn)品圖片 |
Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-ITM2A (bs-9705R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-ITM2A? (bs-9705R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 35 kD
Blank control(blue): 293T Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ITM2A/AF488 Conjugated antibody (bs-9705R-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
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