| 產(chǎn)品編號 | bs-10610R |
| 英文名稱 | DRD1 Rabbit pAb |
| 中文名稱 | 多巴胺受體D1抗體 |
| 別 名 | dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1A; DRD1_HUMAN; DRD 1A. |
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Specific References (1) | bs-10610R has been referenced in 1 publications.
[IF=3.322] Hua-chun Miao. et al. Expression changes of c-Fos and D1R/p-ERK1/2 signal pathways in nucleus accumbens of rats after ketamine abuse. BIOCHEM BIOPH RES CO. 2022 Sep;: IHC, WB ; Rat.
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| 研究領域 | 腫瘤 免疫學 神經(jīng)生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | 65-80 |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Dopamine Receptor D1: 11-100/446 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008] Function: Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. Subunit: Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Tissue Specificity: Detected in caudate, nucleus accumbens and in the olfactory tubercle. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P21728 Gene ID: 1812 Database links: Entrez Gene: 1812 Human Entrez Gene: 13488 Mouse Omim: 126449 Human SwissProt: P21728 Human SwissProt: Q61616 Mouse Unigene: 2624 Human Unigene: 54161 Mouse Unigene: 24039 Rat |
| 產(chǎn)品圖片 |
Sample:A549 Cell(Human)Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 60kD
Sample:
A431 Cell(Human)Lysate at 40 ug
HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 60kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated(bs-10610R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell: SH-SY5Y
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-10610R) on SH-SY5Y(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
