| 產品編號 | bs-17651R |
| 英文名稱 | SPEF1 Rabbit pAb |
| 中文名稱 | 精子鞭毛蛋白1抗體 |
| 別 名 | C20orf28; Calponin homology and microtubule associated protein; CLAMP; SPEF1A; Sperm flagellar 1; Sperm flagellar protein 1. |
| 研究領域 | 細胞生物 免疫學 發(fā)育生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 27 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SPEF1: 181-236/236 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
CLAMP is a 236 amino acid protein that is present in epididymal sperm and localizes to the cell projection, as well as to the cytoplasm. Expressed in lung, brain and testis, CLAMP functions as a microtubule-associated protein that is thought to play a role in microtubule bundling. Human CLAMP exists as two alternatively spliced isoforms and shares a high degree of homology with its mouse counterpart, suggesting a conserved role between species. The gene encoding CLAMP maps to human chromosome 20, which houses over 600 genes and comprises nearly 2% of the human genome. Subcellular Location: Cytoplasm. Cell projection; cilium; flagellum. Cytoplasm; cytoskeleton; cilium axoneme. Note: Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia. SWISS: Q9Y4P9 Gene ID: 25876 Database links: Entrez Gene: 25876 Human Omim: 610674 Human SwissProt: Q9Y4P9 Human Unigene: 72620 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPEF1) Polyclonal Antibody, Unconjugated (bs-17651R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPEF1) Polyclonal Antibody, Unconjugated (bs-17651R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
