| 產品編號 | bs-17781R |
| 英文名稱 | MRPL22 Rabbit pAb |
| 中文名稱 | 線粒體核糖體蛋白L22抗體 |
| 別 名 | 39S ribosomal protein L21; 39S ribosomal protein L21, mitochondrial; 39S ribosomal protein L21, mitochondrial precursor; L21mt; MGC62013; mitochondrial; Mitochondrial ribosomal protein L21; MRP L21; MRP-L21; Mrpl21; RM21_HUMAN. |
| 研究領域 | 細胞生物 免疫學 轉錄調節(jié)因子 線粒體 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Zebrafish,Chicken) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 23 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MRPL22: 41-120/206 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Subcellular Location: Mitochondrion. Similarity: Belongs to the ribosomal protein L22P family. SWISS: Q9NWU5 Gene ID: 29093 Database links: Entrez Gene: 29093 Human Entrez Gene: 216767 Mouse Omim: 611835 Human SwissProt: Q9NWU5 Human SwissProt: Q8BU88 Mouse Unigene: 483924 Human Unigene: 259907 Mouse Unigene: 13421 Rat |
| 產品圖片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-MRPL22 (bs-17781R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
