| 產(chǎn)品編號 | bs-18539R |
| 英文名稱 | Rabbit Anti-CLEC16A antibody |
| 中文名稱 | C型凝集素結構域家族16成員A抗體 |
| 別 名 | Protein CLEC16A; C type lectin domain family 16 member A; CL16A; Gop 1; Gop1; CL16A_HUMAN； KIAA0350; MGC111457. |
| 研究領域 | 細胞生物 免疫學 糖尿病 自然殺傷細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Pig,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 118kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CLEC16A |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the C-type lectin domain containing family. Single nucleotide polymorphisms in introns of this gene have been associated with diabetes mellitus, multiple sclerosis and rheumatoid arthritis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] Subcellular Location: Cell membrane, Cytoplasmic. Tissue Specificity: Almost exclusively expressed in immune cells, including dendritic cells, B-lymphocytes and natural killer cells. DISEASE: Insulin-dependent diabetes mellitus is significantly associated with variation within a 233-kb linkage disequilibrium block on chromosome 16p13 that includes KIAA0350. Three common non-coding variants of KIAA0350 in strong linkage disequilibrium reach genome-wide significance for association with the disease. Similarity: Belongs to the CLEC16A/gop-1 family. SWISS: Q2KHT3 Gene ID: 23274 Database links: Entrez Gene: 23274 Human Entrez Gene: 74374 Mouse Omim: 611303 Human SwissProt: Q2KHT3 Human SwissProt: Q80U30 Mouse |
| 產(chǎn)品圖片 |
Sample: k562 (human)Cell Lysate at 40 ug
Primary: Anti-CLEC16A(bs-18539R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
Sample:
K562(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
U-2OS(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- CLEC16A (bs-18539R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kD
Observed band size: 118 kD
Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37∑C for 30min; Antibody incubation with (CLEC16A) Polyclonal Antibody, Unconjugated (bs-18539R) at 1:500 overnight at 4∑C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(bs-18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-CLEC16A Polyclonal Antibody, Unconjugated(bs-18539R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
