| 產(chǎn)品編號 | bs-19936R |
| 英文名稱 | RUVBL1 Rabbit pAb |
| 中文名稱 | RUVBL1抗體 |
| 別 名 | 49 kDa TATA box binding protein interacting protein; 49 kDa TATA box-binding protein-interacting protein; 49 kDa TBP interacting protein; 49 kDa TBP-interacting protein; 54 kDa erythrocyte cytosolic protein; ECP-54; ECP54; ERYTHROCYTE CYTOSOLIC PROTEIN, 54-KD; INO80 complex subunit H; NMP 238; Nuclear matrix protein 238; Pontin 52; PONTIN; RuvB like 1; RuvB-like 1; RUVB1_HUMAN; RVB1; TAP54 alpha; TAP54-alpha; TIP49; TIP49A; TIP60 associated protein 54 alpha; TIP60-associated protein 54-alpha. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞凋亡 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RUVBL1: 101-200/456 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] Function: Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May be able to bind plasminogen at cell surface and enhance plasminogen activation. Essential for cell proliferation. Subcellular Location: Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells. Tissue Specificity: Ubiquitously expressed with high expression in heart, skeletal muscle and testis. Similarity: Belongs to the ruvB family. SWISS: Q9Y265 Gene ID: 8607 Database links: Entrez Gene: 8607 Human Entrez Gene: 56505 Mouse Omim: 603449 Human SwissProt: Q9Y265 Human SwissProt: P60122 Mouse Unigene: 272822 Human Unigene: 42195 Mouse Unigene: 86410 Rat |
| 產(chǎn)品圖片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-TIP49A (bs-19936R) at 1/500 dilution
Anti-TIP49A (bs-19936R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TIP49A) Polyclonal Antibody, Unconjugated (bs-19936R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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