| 產(chǎn)品編號 | bs-4429R |
| 英文名稱 | PTPLB Rabbit pAb |
| 中文名稱 | 蛋白酪氨酸磷酸酶PTPLB抗體 |
| 別 名 | 3 hydroxyacyl CoA dehydratase 2; 3-hydroxyacyl-CoA dehydratase 2; HACD2; HACD2_HUMAN; Protein tyrosine phosphatase like(proline instead of catalytic arginine) member b; Protein tyrosine phosphatase like member B; Protein-tyrosine phosphatase-like member B; PTPLB; Very-long-chain(3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 2. |
| 研究領(lǐng)域 | 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 28 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PTPLB: 181-254/254 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene can catalyze the third step (dehydration) in the conversion of long chain fatty acids to very long chain fatty acids. The encoded protein localizes to the endoplasmic reticulum membrane. [provided by RefSeq, Jul 2016] Function: Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Highly expressed in testis, spleen, prostate, colon and heart, followed by moderate expression in thymus, ovary, small intestine, peripheral blood leukocytes, liver, skeletal muscle and pancreas. Weakly detected in kidney, placenta, brain and lung. Similarity: Belongs to the very long-chain fatty acids dehydratase HACD family. SWISS: Q6Y1H2 Gene ID: 201562 Database links: Entrez Gene: 201562 Human Entrez Gene: 70757 Mouse SwissProt: Q6Y1H2 Human SwissProt: Q9D3B1 Mouse Unigene: 705480 Human Unigene: 27286 Mouse |
| 產(chǎn)品圖片 |
Sample:
Du145(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (bs-4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (bs-4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPLB) Polyclonal Antibody, Unconjugated (bs-4429R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
