| 產品編號 | bs-10523R |
| 英文名稱 | Rabbit Anti-Cathepsin L(heavy chain) antibody |
| 中文名稱 | 組織蛋白酶L抗體 |
| 別 名 | Cathepsin L; Major Excreted Protein; Cathepsin L1; EC 3.4.22.15; CTSL1; MEP; EC 3.4.22; CATL; CATL1_MOUSE; |
| 研究領域 | 腫瘤 細胞生物 免疫學 激酶和磷酸酶 合成與降解 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Cathepsin L: 101-200/334 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012] Function: Important for the overall degradation of proteins in lysosomes. Subunit: Dimer of a heavy and a light chain linked by disulfide bonds. Subcellular Location: Lysosome. Similarity: Belongs to the peptidase C1 family. SWISS: P06797 Gene ID: 13039 Database links: Entrez Gene: 13039 Mouse SwissProt: P06797 Mouse Unigene: 930 Mouse Unigene: 1294 Rat 合成與降解（Synthesis and Degradation） 組織蛋白酶L(cathepsin L CL)屬于木瓜蛋白酶家族中的半胱氨酸蛋白水解酶，以酶原的形式貯存于溶酶體中. 參與許多特殊的生理過程，如激素原的激活、抗原呈遞、組織器官的發(fā)育等。 在病理狀態(tài)下，因各種原因所致的細胞損傷(如病原微生物、炎癥因子、氧化應激等)可降解細胞成分或細胞間質基質成分，包括層粘素、IV型膠原纖維及纖維連接素等，與人類許多疾病如腫瘤的浸潤與轉移、關節(jié)炎、骨質疏松、阿爾茨海姆病、多發(fā)性硬化癥及其他慢性炎癥性疾病有關. |
| 產品圖片 |
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Mouse Kidney tissue lysates
Primary: Anti-Cathepsin L(heavy chain) (bs-10523R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/37 kDa
Observed band size: 40 kDa
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cathepsin L(heavy chain)) Polyclonal Antibody, Unconjugated (bs-10523R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cathepsin L) Polyclonal Antibody, Unconjugated (bs-10523R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
