| 產(chǎn)品編號 | bs-23393R |
| 英文名稱 | GCLC Rabbit pAb |
| 中文名稱 | γ谷氨酰半胱氨酸合成酶抗體 |
| 別 名 | gamma ECS; gamma GCS; gamma glutamylcysteine synthetase; gamma-ECS; gamma-glutamylcysteine synthetase; GCL; GCLC; GCS; GCS Heavy Chain antibody GLCL; GLCLC; GLCLR; Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase(gamma glutamylcysteine synthetase) regulatory(30.8kD); Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase modifier subunit; Glutamate cysteine ligase regulatory protein; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN. |
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Specific References (5) | bs-23393R has been referenced in 5 publications.
[IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268. WB ; Human.
[IF=4.253] Mengru Li. et al. The leaves of Scutellaria baicalensis Georgi attenuate brain aging in D-galactose-induced rats via regulating glutamate metabolism and Nrf2 signaling pathway. EXP GERONTOL. 2022 Dec;170:111978 WB ; Rat.
[IF=4.014] Zhang Kaixin. et al. MiR-129-3p regulates ferroptosis in the liver of Selenium-deficient broilers by targeting SLC7A11. POULTRY SCIENCE. 2022 Oct;:102271 WB ; Chicken.
[IF=3.909] Li ZP et al. Protective roles of Amanita caesarea polysaccharides against Alzheimer's disease via Nrf2 pathway. (2018) Int J Biol Macromol.121:29-37. WB ; Mouse.
[IF=2.767] Dezhuan Da. et al. Glutamate-cysteine ligase catalytic and its modifier function as novel immunotargets in gastric adenocarcinoma. Asian J Surg. 2022 Mar;: IHC ; Other.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Zebrafish,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 73 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GCLC: 351-450/637 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. Defects in GCLC are the cause of hemolytic anemia. Subunit: Heterodimer of a catalytic heavy chain and a regulatory light chain. DISEASE: Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. Similarity: Belongs to the glutamate--cysteine ligase type 3 family. SWISS: P48506 Gene ID: 2729 Database links: Entrez Gene: 2729 Human Entrez Gene: 14629 Mouse Omim: 606857 Human SwissProt: P48506 Human SwissProt: P97494 Mouse Unigene: 654465 Human Unigene: 89888 Mouse Unigene: 8365 Rat |
| 產(chǎn)品圖片 |
Sample:
Kidney (Mouse) Lysate at 40 ug
Kidney (Rat) Lysate at 40 ug
Primary: Anti-GCLC (bs-23393R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kD
Observed band size: 73 kD
Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Liver tissue lysates
Lane 3: Human HepG2 cell lysates
Primary: Anti-GCLC (bs-23393R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (bs-23393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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