| 產(chǎn)品編號(hào) | bsm-60564R |
| 英文名稱 | GRK2 Recombinant Rabbit mAb |
| 中文名稱 | |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | D3F9 |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:5000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG/Kappa |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | 特異性磷酸化β-腎上腺素能受體和密切相關(guān)受體的激動(dòng)劑占據(jù)形式，可能誘導(dǎo)它們脫敏。LPAR1信號(hào)的關(guān)鍵調(diào)節(jié)器。與RALA競(jìng)爭(zhēng)與LPAR1的結(jié)合，從而影響受體的信號(hào)特性。以非磷酸化的方式使LPAR1和LPAR2脫敏。正調(diào)控纖毛平滑（SMO）依賴的刺猬（Hh）信號(hào)通路，通過(guò)促進(jìn)SMO進(jìn)入纖毛和刺激SMO活性。抑制氣道平滑肌對(duì)藍(lán)光的反應(yīng)。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:5000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: Mouse brain, Rat brain
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 80 kDa
Observed MW: 80 kDa
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-GRK2 antibody (bs-60564R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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