| 產(chǎn)品編號 | bsm-30095M-mFV540 |
| 英文名稱 | CD45 |
| 中文名稱 | mFV540標(biāo)記的小鼠抗人CD45單克隆抗體 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | HI30 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | Flow-Cyt=10ul/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 免 疫 原 | Recombinant human CD45 |
| 亞 型 | Mouse IgG1, k |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M PBS (pH7.4), 0.5% BSA, 0.02% Proclin300. |
| 保存條件 | Store at 2-8℃. Protect from light. Avoid freezing. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling Function: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. Subunit: Binds GANAB and PRKCSH. Interacts with SKAP1. Interacts with DPP4; the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Contains 2 tyrosine-protein phosphatase domains. Subcellular Location: Membrane; Single-pass type I membrane protein. Membrane raft. Note=Colocalized with DPP4 in membrane rafts. Post-translational modifications: Heavily N- and O-glycosylated. DISEASE: Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. Similarity: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains. Database links: CD45在活化信號轉(zhuǎn)導(dǎo)中起到調(diào)節(jié)作用，在確定CD45為一種PTPase之前就已證實(shí)了CD45參于細(xì)胞的活化和生長調(diào)節(jié)。 抗CD45抗體可以抑制PHA或CD3交聯(lián)所介導(dǎo)的T細(xì)胞增殖，還可抑制NK或細(xì)胞毒性T細(xì)胞對靶細(xì)胞的殺傷，抑制經(jīng)CD2、CD3以及CD8膜分子介導(dǎo)的信號轉(zhuǎn)導(dǎo)作用。 |
