| 產(chǎn)品編號(hào) | bs-3789R-Gold |
| 英文名稱1 | Rabbit Anti-Perilipin A/Gold Conjugated antibody |
| 中文名稱 | 膠體金標(biāo)記的脂滴包被蛋白Perilipin-A抗體 |
| 別 名 | Lipid droplet associated protein; Lipid droplet-associated protein; PERI; Perilipin; Perilipin-1; Perilipin 1; PerilipinA; Perilipin-A; PLIN; PLIN1; PLIN1_HUMAN. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說(shuō) 明 書(shū) | 100ul（10nm 15nm 35nm） |
| 研究領(lǐng)域 | 心血管 免疫學(xué) 合成與降解 內(nèi)分泌病 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Sheep, ) |
| 產(chǎn)品應(yīng)用 | IEM=1:20-200 ICA=1:20-200 ChIP=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 0.4mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Perilipin-1 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.02M TBS(pH8.2) with 1% BSA, 0.03% Proclin300. |
| 保存條件 | Store at 2-8 oC for 3-6 months. Avoid repeated freeze/thaw cycles. |
| 產(chǎn)品介紹 |
background: Perilipins, members of the PAT protein family (named after lipid droplet proteins Perilipin, Adipophilin, and TIP47) are found exclusively at the surface of lipid droplets in adipocytes and steroidogenic cells. They have been suggested to function as regulators of lipolysis and triacylglycerol storage within adipose tissue. Four distinct isoforms ranging from perilipin A (57 kDa) to perilipin D (26 kDa) have been identified and they share an identical amino terminal sequences, and contain 2–6 consensus protein kinase A (PKA) phosphorylation sites. Perilipin C and D have been detected only in steroidogenic cells. Perilipin A is the most abundant form on the lipid droplets of adipocytes. The phosphorylation of perilipin by PKA, which is accompanied by the phosphorylation and translocation of hormone-sensitive lipase from the cytosol to the lipid droplets, promotes lipolysis. There is evidence for the presence of perilipin A in atheroma plaques suggesting that the protein may be involved in the development of therosclerosis by controlling as in adipocytes the hydrolysis of stored lipids. Function: Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Subunit: Interacts with ABHD5. Subcellular Location: Lipid droplet. Note=Lipid droplet surface-associated. Tissue Specificity: Adipocytes. Post-translational modifications: Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. DISEASE: Defects in PLIN1 are the cause of familial partial lipodystrophy type 4 (FPLD4) [MIM:613877]. FPLD4 is a form of lipodystrophy characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension. Similarity: Belongs to the perilipin family. Database links: Entrez Gene: 5346 Human Entrez Gene: 103968 Mouse Omim: 170290 Human SwissProt: O60240 Human SwissProt: Q8CGN5 Mouse Unigene: 103253 Human Unigene: 254917 Mouse Unigene: 9737 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ????脂肪細(xì)胞中貯存的甘油三酯的水解是人和動(dòng)物體內(nèi)精細(xì)調(diào)節(jié)的動(dòng)態(tài)平衡過(guò)程，與維持機(jī)體能量的動(dòng)態(tài)平衡和代謝正常密切相關(guān)。Perilipin A是成熟脂肪細(xì)胞中脂滴膜上表達(dá)量較多的一種結(jié)構(gòu)和功能蛋白，是多條脂解通路的終末靶點(diǎn)之一，對(duì)脂肪細(xì)胞的脂肪分解起著關(guān)鍵的調(diào)控作用. ????perilipin可能在脂肪分解調(diào)控中起到“分子開(kāi)關(guān)”的作用。蛋白激酶A（PKA）、細(xì)胞外信號(hào)調(diào)節(jié)激酶（ERK）等信號(hào)轉(zhuǎn)導(dǎo)通路參與了脂肪分解。腫瘤壞死因子僅（TNFα）、過(guò)氧化物酶體增殖物激活受體γ（PPAγ）激動(dòng)劑、瘦素（leptin）均可以影響perilipin的表達(dá)。近年來(lái)研究，perilipin可通過(guò)蛋白酶體途徑來(lái)調(diào)節(jié)其蛋白量的表達(dá)。脂肪分解調(diào)控中的關(guān)鍵蛋白perilipin對(duì)2型糖尿病、肥胖、動(dòng)脈粥樣硬化等多種代謝性疾病及心血管疾病相關(guān)聯(lián)。 |
