| 產(chǎn)品編號 | bs-13054R-HRP |
| 英文名稱 | Rabbit Anti-eEF1A2/HRP Conjugated antibody |
| 中文名稱 | 辣根過氧化物酶標記的翻譯延伸因子EF-1 α2抗體 |
| 別 名 | eEF1A 2; eEF1A-2; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-2; EF1A; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 2; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; HS1; OTTHUMP00000031776; Statin like; Statin S1; Statin-S1; STN; STNL. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 神經(jīng)生物學 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, Zebrafish, ) |
| 產(chǎn)品應用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human eEF1A2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Elongation factor 1 alpha 2 (EF-1 alpha2) is a eukaryotic protein translation factor that is expressed in terminally differentiated cells of skeletal muscle, heart, and certain areas of the brain. Along with protein synthesis, EF-1 alpha2 also plays a role in cytoskeletal remodelling and apoptosis. The gene encoding for EF-1 alpha2 maps to chromosome 20q13. Wasted (wst) refers to a spontaneous autosomal recessive mutation in which this gene is deleted, and it leads to tremors and disturbances of gait shortly after weaning, followed by motor neuron degeneration, paralysis, and death by about 28 days in mice. EF-1 alpha2 has the ability to transform mammalian cells and is highly expressed in tumors of the ovary, breast, and lung, thereby proving a good candidate for an oncogene. In addition to this, EF-1 alpha2 enhances focus formation, allows anchorage-independent growth, and decreases the doubling time of rodent fibroblasts. Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Subunit: Monomer Subcellular Location: Nucleus. Tissue Specificity: Brain, heart, and skeletal muscle. Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily. Database links: Entrez Gene: 1917 Human Entrez Gene: 13628 Mouse Entrez Gene: 101109898 Sheep Entrez Gene: 380550 Xenopus laevis Omim: 602959 Human SwissProt: Q05639 Human SwissProt: P62631 Mouse Unigene: 433839 Human Unigene: 2645 Mouse Unigene: 9764 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
