| 產(chǎn)品編號(hào) | bs-10140R-BF350 |
| 英文名稱 | Rabbit Anti-PDE4A/BF350 Conjugated antibody |
| 中文名稱 | BF350標(biāo)記的磷酸二酯酶4A抗體 |
| 別 名 | 5''-cyclic phosphodiesterase 4A; cAMP specific 3 5 cyclic phosphodiesterase 4A; cAMP specific phosphodiesterase; cAMP-specific 3''; cAMP-specific 3',5'-cyclic phosphodiesterase 4A; DPDE2; dunce like phosphodiesterase E2; PDE 4A; PDE4; PDE46; PDE4A; PDE4A_HUMAN; PDE4A11; Phosphodiesterase 4A; phosphodiesterase 4A cAMP specific; Phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog; Phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4; phosphodiesterase type 4A11. |
| 規(guī)格價(jià)格 | 100ul/2980元 購(gòu)買 大包裝/詢價(jià) |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat, (predicted: Human, Mouse, Pig, Cow, Horse, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 98kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDE4A |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲(chǔ) 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. In brain, more than 90% of cAMP formed by the stimulation of GPCRs is hydrolyzed by PDE4 enzymes. PDE4 enzymes are also important molecular targets for a variety of therapeutic agents like antidepressants, anti-asthmatics, and anti-inflammatory drugs. PDE4 family comprises 4 genes (PDE4A, B, C and D); each exhibiting multiple isozymes due to alternate splicing that leads to a larger number of distinct PDE4 variants. Members of the PDE4 family are regulated/activated by phosphorylation/dephosphorylation by cAMP-dependent protein kinase A and phosphatases. Protein-protein interactions and cellular trafficking of PDE4A enzymes play an important role in cAMP compartmentalization and cAMP-dependent signaling. In brain members of the PDE4A, B and D family are associated with GPCRs (adrenergic and dopaminergic) signaling. There are 5 isoforms. Function: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Subunit: Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2. Subcellular Location: Isoform 1: Cytoplasm, perinuclear region. Isoform 2: Cytoplasm, perinuclear region. Cell projection, ruffle membrane. Isoform 4: Membrane; Peripheral membrane protein. Note=Isoform 4 has propensity for association with membranes. Isoform 6: Cytoplasm, perinuclear region. Isoform 7: Cytoplasm. Membrane. Note=Predominantly cytoplasmic. Tissue Specificity: Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level). Post-translational modifications: Phosphorylation by MAPKAPK2 its activation through PKA phosphorylation. Phosphorylated at Ser-686 and Ser-688 when expressed in S.frugiperda cells. Isoform 2 and isoform 7 are activated by phosphorylation at Ser-119 and Ser-123 respectively by PKA. Proteolytically cleaved by caspase-3. DISEASE: Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum (at protein level). Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Database links: Entrez Gene: 5141 Human Entrez Gene: 18577 Mouse Omim: 600126 Human SwissProt: P27815 Human SwissProt: O89084 Mouse Unigene: 89901 Human Unigene: 191749 Mouse Unigene: 91357 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
